Azure-c600-Infrared-Visible Fluorescent Western Blot Imaging System

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The Ultimate Western Blot Imaging System – Capture Western blot images any way you want.  Quantify two proteins on the same blot

  • Two-channel IR direct detection – Laser diodes at 660nm and 785nm enable better sensitivity. IR detection reduces background noise for sensitive detection of low abundance proteins.
  • Three Channel RGB Excitation – For Cy5/Cy3/Cy2 applications –allows quantitation of two proteins (or modifications) and a loading control without stripping and re-probing
  • Image Chemiluminescent Westerns delivers ultimate sensitivity for low abundance proteins.
  • Capture all your gel and blot images on a single instrument. DNA, protein, colorimetric or photometric gels with your choice of stains or dyes.

 

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A Total Solution for Western Blots and Gel Documentation

  • Lasers for the excitation of IR 700/800 dyes,
  • excitation channels at 628 nm, 526 nm, 460 nm for Cy® dyes or Alexa Fluor® dyes,
  • Picogram Detection of Proteins with Chemiluminescent Westerns,
  • 470 nm excitation for safe dyes,
  • 302/365 nm UV,
  •  and white light  make the Azure c600 a multichannel, multimodal imager.

Imaging system supports

  • Blue Exited DNA Dyes
  • Visible Imaging
  • UV Fluorescence.
  • Chemiluminiscence
  • Visible fluorescence Western Imaging
  • NIR Fluorescence Western Imaging

Smart Imaging Technology

  • No Focusing: Regardless of the size of your gel, all images are in focus without adjusting the focus.
  • No Positioning: Perfect images no matter where you place the sample in the imager.
  • Automatic Imaging: Use your choice of dyes or stains and the system automatically selects the light source and filters for you. UV for Ethidium bromide-stained DNA gels, blue light for SYBR® Safe or similar dyes, white light for Silver stain or Coumassie Blue.

Touch Screen tablet computer

  • WIN 8 tablet computer controls the system with the touch of a finger and enables compatibility with existing networks, as well as easy setup
  • Bluetooth and wireless compatible, or USB to LAN compatible
  • 10.1” touch screen
  • 2GB of storage for short term data saving

Powerful camera and optics

  • High Resolution Camera: 8.3 MP CCD camera (3326X2504), with 5.4 micron pixels and 16 bit A/D
  • Deep Peltier cooling: -50°C from ambient, regulated, eliminating electronic noise in chemiluminescent images
  • Lens: Motorized 0.95 fixed lens for fast sensitive image capture, and short exposure times
  • Dual-Focus Technology: Image samples in perfect focus, and with optimal lens settings, without having to touch the camera.
  • Fluorescent and Chemiluminescent Protein array imaging

Versatile Darkroom

  • Light Tight Darkroom: Darkroom with automated software controls.
  • Multiple Filter Positions: 7 position motorized filter wheel with Orange red green blue, 700, 800 filters.
  • EPI White Lights: Provide overhead illumination for white light imaging.
  • EPI Blue LED at 470 nm for Blue-Excited DNA Dyes: Excitation for dyes like SYBR® Safe, enabling the user to use dyes that are less harmful to the DNA sample, and to the environment.
  • Visible Dye Imaging: Transilluminated white light imaging source for colorimetric stains such as Coomassie blue or similar.
  • Dual-Wavelength UV: UV illumination with peaks at 302 and 365 nm. Compatible with a wide range of dyes including ethidium bromide, SYBR® green, SYBR gold, SYPRO® orange, fluorescein, RadiantRed®, Texas Red®, SYPRO Red.
  • Safety Lock: Safety interlock to prevent accidental UV exposure. automatically shuts off UV light when door is open
  • Band Excision: Mode Easily excise your DNA bands from an agarose gel with our Band Excision mode. Designed to allow the user to cut bands out of their gel with the system door open, it also turns off the UV light after 5 minutes, ensuring the light is not accidentally left on.
  • Chemi Blot shelf: Auto detection of chemi shelf, for automatic focus adjustment.
  • RGB LEDs : Excitation at 460, 526, and 628nm for Cy2/ Cy3/ Cy5 or similar
  • Laser diodes: 660nm and 785nm for Narrow band excitation enables NIR applications with better sensitivity  than LEDs or white sources.
  • 3 USB Ports: Connect to a drive or network, or attach a thermal printer.

cSeries Capture software

  • Unique GUI for each applications, that requires no focusing or other system adjustments
  • Bin modes for chemi include 2X2, 3X3, 4X4 and 5X5 for enhanced chemiluminescence imaging
  • Gallery mode enables basic editing and saving, and file opening
  • Custom mode for full flexibility and customized imaging protocols.
  • View images in multichannel or single channel mode
  • User manual as PDF in software
  • Image output 16 bit Tiff, Jpeg

 

AzureSpot Analysis Software

Advanced Analysis for 1D Gels and Blots

  •  AzureSpot helps you interpret your data easily and accurately. Analyze your gels and blots in either manual or automatic modes.
  • AzureSpot can automatically detect lanes and bands, even on distorted gels, and apply your selected method of background correction.
  • Visualize intensity plots by lane (sample) and channel (probe) to get detailed information about your proteins of interest.
  • The integrated tutorial helps you get the most information out of your analyses. Generate a PDF report of your experiment when you are done. You can also export your data in many formats to other applications (e.g. Excel) for further analysis.

AzureSpot can

  • Perform 2D densitometry
  • Automatically detect lanes and bands
  • Correct for background
  • Calculate molecular weights
  • Quantify fluorescent signals
  • Annotate your results
  • Visualize multiplex analyses
  • Export reports

Product Features

  • Integrated tutorial
  • PDF report generator
  • Lane creation tools
  • Molecular size/pI calibration
  • Quantity calibration toolbox
  • Module for easy shape selection/deselection
  • Wide range of data fields to display in measurements
  • Annotation tools for comments and highlighting of image

Flexibility “Safe” Dye Detection – Blue Light gel imaging

  • An alternative to ethidium bromide, less-harmful ‘Safe’ dyes can be imaged with the EPI Blue LEDs standard in the system.

Protein Analysis – White Light imaging

  • Protein gels stained with Coomassie blue or silver stain can easily be imaged using the visible light application.

DNA Detection with Ethidium Bromide – UV gel imaging

  • With the a dual-wavelength 302 nm and 365 nm UV transilluminator, images of ethidium bromide-stained DNA gels can be captured in a fraction of a second.

Picogram Detection of Proteins with Chemiluminescent Westerns Imaging

  • With High resolution, deeply cooled camera and 0.95 fast lens technology provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis. Forget the hassles of film and darkroom chemicals – just place your sample in the system and capture an image with the click of a button.

Visible Fluorescence Detection- Quantify three proteins on a single blot

  • C600 have powerful LEDs compatible with Cy5, Cy3 and Cy2 to carry out multiplex blots detecting 2 or 3 different proteins using 2 0r 3 different colours.

Infrared Laser Excitation for Quantitative Western Blot Imaging in the NIR

  •  Quantify two proteins on the same blot without stripping and re-probing.  C500 laser technology offers two IR detection channels enabling a user to image more than one protein in assay. Imaging with Infrared dyes offers signal stability and low background fluorescence for sensitive detection of low abundance proteins. Additionally, imaging with NIR dyes allows you to study multiple proteins in a blot, even if those proteins overlap in molecular weight.
Instrument Type Azure c600
Camera 8.3 MP
Cooling -50°C regulated cooling
Lens F0.95
Image Bit Depth 16 bit
Trans White Light Yes
UV 302 nm & 365 nm Yes
EPI Blue LED Yes
EPI White Yes
EPI RGB LEDs Yes, blue (457nm), green (530nm), and red (628nm)
Chemiluminesence Yes
Visible Fluorescent Westerns Yes
NIR Westerns Yes
Compatible dyes/substrates/kits/labels:

 

The Azure c600 is an open platform. A sample list of compatible dyes is listed below.

  • Multicolor Western Kits :Dylight, ECL Plex
  • Fluorescence: Cy2, Cy3,cy5,IR 700, IR 800
  • Stains/Dyes: Deep Purple, SYBR Green SYPRO Orange, Syptro Ruby, Ethidium Bromide, SYBR Safe, others
  • Chemiluminescence kits and labels: HRP, AP, ECL, ECL Plus, ECL Advance, SuperSignal West Pico
  • Trans UV Fluorescence: Ethidium bromide, SYPRO Ruby
  • EPI Blue fluorescence: SYBR Safe dyes
  • White light transilluminattions: Coomassie blue, silver stain, X-ray film
Filter Wheel 7 position motorized filter wheel
Computer Tablet computer; Option to control system through external PC
External USB Ports 3
Direct Connectivity to Thermal Printer Yes
Upgradeability Yes
Analysis Software Yes
Field of View 20 X 15 cm
Footprint (w X H X D) 38 X 44 X 36 cm

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