DS-11FX Microvolume Spectrophotometer / Fluorometer

ds-11fx
DeNovix product-family
model-choices
dynamic_range
denovix-assay-detection

The most sensitive 1µL UV-Vis absorbance combined with the most flexible and precise fluorometer in one compact, maintenance-free instrument.

  • 1µl Full spectrum UV-Vis (190-840 nm)
  • Four Channel Fluorescence ( (UV, Blue, Green, Red) 
  • Android™ OS with EasyApps® (no PC)
  • SmartQC Sample Contamination Guidance
  • Export to Network, e-mail, Wi-Fi, USB, LIMS, Printers

 

Download Brochure

World’s first Android™ Micro-Volume UV-Vis Spectrophotometer / Fluorometer combine UV-Vis absorbance and fluorescence methods in one compact instrument. (1uL UV-Vis + fluorescence)

Compact, Stand-Alone Design

  • 20 x 33 cm
  • Weight: 2 kg
  • No laptop
  • Saves space

Full Spectrum Analysis

  •  Denovix DS-11FX Spectrophotometer quantifies proteins and nucleic acids via UV-Vis absorbance and requires only 0.5 – 1.0 µl samples. Instantly view results on screen including full spectrum output, concentrations, purity ratios etc. Suited for any absorbance applications within its 190 to 840nm range.  Just pipette and measure. It’s that simple. Two seconds DNA, RNA, and Protein quantification.
  • DS-11 FX can rapidly quantify seven orders of magnitude of nucleic acids and protein samples utilizing your choice of UV-Vis or Fluorescent modes.

The Only All-In-One Absorbance and Fluorescence Design

  • The choice of microvolume absorbance and fluorescence methods delivers unmatched flexibility and dynamic range. Coupled with the DeNovix dsDNA quantification assays, a range of 0.5 pg/µL to 37500 ng/µL can be rapidly measured
  • DS- 11 FX with Microvolume Mode (Smartpath Technology with Bridge Testing ) can measure low and high concentrations (0.75 to 37,500 ng/µL dsDNA and 0.04 – 1125 mg/mL BSA)
  • DS-11 FX with Fluorometer Mode (Fluorescence Measurements with 0.5ml PCR tube) can quantify nucleic acids (0.5 pg/uL of dsDNA) and proteins using four fluorescent channels and a choice of common assays which are built into measurement apps.

Total Measurement Confidence
The patented* DS-11 FX combines unique hardware and software features to deliver unmatched confidence in sample accuracy, performance and reliability.

  • SmartPath®Technology – real time, precise pathlength control delivered every time
  • Exceptional for proteins– BridgeTesting® eliminates broken sample columns common on other platforms
  • Calibration and maintenance free –no measurement drift, no service down-time. Accurate results every time.
  • Industry-leading 3-year warranty

Contamination Alerts

  • SmartQC monitors every sample measured for the presence of common contaminants, highlights these samples to the user and advises on potential issues. SmartQC also solves the problem of samples being left to dry on the measurement surface by monitoring the sample measurement and cleaning process and warning when the sample has not been removed.

Intuitive and Easy.

  •  DS-11 FX is simple to use and fast to learn using DeNovix EasyApps®. The software is pre-installed on a AndroidTM operating system that features an HD, glove-compatible touchscreen. Pinch/ Zoom / Swipe, 11 languages built –in, 32 GB memory and Audio,  Every instrument is ready for absorbance and fluorescence measurements right out of the box – no PC set-up or software installation.

 Connect to Your Results.

  • Built-in Wi-Fi, Ethernet and USB allow easy export of data you want in the format you need. Email a csv or color jpg of your results…save data to LIMS or network drives…print results or graphs to network and label printers…and more! Password protected accounts

Four color choices

  • Cutting Edge and Tech Savvy– how about the modern Arctic White option for your lab
  • Resourceful and Dynamic – then the brilliant Brazilian Blue fits your style!
  • Exciting and Passionate about your research- then the Fire Red might be your best choice!
  • Bold and Innovative in your approach- perhaps the contemporary Tungsten Silver is best for you!

Microvolume Mode: Smartpath Technology with Bridge Testing

  • SmartPath® Technology is Denovix patent pending design to precisely control microvolume pathlength in real-time. SmartPath® ensures the DS-11FX is always in calibration – no drift and no service down-time.
  • SmartPath® technology controls the pathlength in real time ensuring accurate precise measurements. Bridge Testing™ is Denovix propriety process to compress and monitor a sample during measurements to ensure the liquid column is intact. Measurements are accurate and precise for 1µL, even for difficult to measure samples like protein.

Best in Class 1uL Sample Lower Detection Limits!

DeNovix Spectrophotometer set the industry standard for the lower detection limits of 0.5 -1.0 uL UV-Vis sample analysis. Detect to 0.75 ng/uL dsDNA, 0.60 ng/uL RNA or 0.04 mg/mL BSA.

Always Calibrated

  • The DS-11FX does not rely on fixed path length calibrations and therefore does not require regular calibration checks. There is no downtime or expense required for routine calibration like many other spectrophotometers. DeNovix offers a LC-NA verification standard for labs that may need to verify proper operation from time to time in accordance with their own quality standards.

Bridge Testing®

  • DeNovix software includes a proprietary algorithm to test for an unbridged condition in the primary nucleic acid and protein quantification apps. This element of the SmartPath® algorithm not only detects the broken liquid column but compensates to ensure accurate results with no user action required.

Truly 1.0 uL Sample Volumes

  • A key feature of the DS-11FX is that the longest path length used (0.5 mm path) is shorter than the 1.0 mm path used by other microvolume spectrophotometers. This enables the DS-11FX to truly measure 1.0 uL samples, virtually eliminating the possibility of erroneous results due to sample column breakage.

Automatic Pathlength Adjustment

The DS-11FX uses real-time absorbance data to determine the best path length (ranging from 0.03-0.5 mm) to use for each measurement. Ultra-short pathlengths enable highest abs. measurements

           0.015 to 750 AU (1 cm equivalent)

           0.75 to 37,500 ng/ml for dsDNA

Ultra High Absorbance

  • The DS-11FX can measure samples with absorbance values as high as 750 absorbance units (at a 1 cm equivalent path length). This translates into 1125 mg/ml of BSA protein or 37,500 ng/uL of dsDNA. This ultra high absorbance capability is achieved by using a remarkably small path length of 0.02mm enabled by our SmartPath® Technology. Even at this short path length, typical CV of measurements is less than 3%.

Superb Precision

  • The reproducibility of measurements on the DS-11FX is an important performance feature. For example, replicates of 500 ng/ul dsDNA samples typically result in CV’s of less than 1%.

Fluorescence Mode: 0.5ml thin –walled PCR tubes, Fluorescence measurements

The DS-11 FX provides researchers with ease-of-use and flexibility like no other fluorometer available. Four fluorescent channels allow scientists to utilize an expanded selection of fluorophores.

Assay Independence

  • Choose the assay you wish to work with. DeNovix EasyApps® are pre-configured with a large selection of commercially available fluorometric assays. Easily create standard curves and quantify dsDNA, RNA and protein using assays designed for Qubit® and QuantusTM platforms. Choices include Qubit®dsDNA BR & HS, QuantiFluor®DNA and RNA, Quant-iT PicoGreen®and many others.

Multiple Channels

  • DeNovix Fluorometers include four emission and excitation channels to provide scientists the flexibility to use the fluorophore and detection ranges of your choice. Choose from UV (375 nm), Blue (470 nm), Green (525 nm) or Red (635 nm) excitation sources.

Assays included in DeNovix EasyApps®
Choose from one of these pre-programmed assays, or create your own!

  • Fluoro dsDNA: DeNovix dsDNA Broad Range, DeNovix dsDNA High Sensitivity, DeNovix dsDNA Ultra High Sensitivity, Quant-iT PicoGreen® , Quant-iT  dsDNA HS, Quant-iT  dsDNA BR, Qubit®dsDNA BR, Qubit®dsDNA HS, Quantifluor dsDNA and Quantifluor One dsDNA
  • Fluoro RNA: Quant-iT Ribogreen, Quant-iT RNA BR, Quant-iT RNA HS, Qubit® RNA BR, Qubit® RNA HS, Quantifluor RNA, Qannt-iT microRNA, Qubit® MicroRNA
  •  Fluoro ssDNA: Quant-iT OliGreen, Qubit® ssDNA, Quantifluor ssDNA
  • Fluoro Protein: Quant-iT Protein, Qubit® Protein

EasyApps® Software

DeNovix DS 11 FX Spectrophotometer/ Fluorometer is compact, stand-alone instrument with intuitive, EasyApps® for quantifying DNA, RNA and Protein. Pre-configured apps ensure optimized workflows while powerful Custom Formula Methods app enables researchers to quickly create and save new methods.

Key Features

  • Intuitive app Icons for quick recognition
  • Familiar Android navigation touch gestures and functional icons
  • Fluid pinch, zoom glove compatible touch interface
  • AutoRun option for streamlined operation
  • Optimized screens for reports and graph overlays
  • Password protected accounts for project and data management
  • Absorbance represented as 1 cm equivalent for all apps- no confusion between applications
  • Sample name field is key-focused for easy barcode reader use
  • All data is automatically archived and easily exported in tab-delimited format

Nucleic: dsDNA, RNA, ssDNA (Absorbance and Fluorescence)

Key Features: Absorbance

  • Separate preconfigured apps for optimized workflow
  • Preconfigured and user defined factors for ssDNA ensures appropriate concentration calculations
  • High absorbance capability: up to 37,500
  •  ng/ul for dsDNA
  • Nucleotides absorb at 260nm
  • Absorbance * factor = concentration (ng/μl)
  • 260/280 and 260/230 ratios reported
  • 11 Preconfigured dyes and fluorophores for Microarray
  • Option to add an unlimited number of new dyes for quick recall in Microarray

 Key Features: Fluorescence

  • Preconfigured commonly used DeNovix dsDNA, Qubit®, Quant-itTM and Qunatifluor® assays
  • Easy configuration of additional assays
  • Single or multi-replicate standard curve options
  • User control of  line fit, trend line analysis, subtraction of blank
  • User control of assay and reported sample concentration units
  • Save and re-use curves and lists of standard concentrations

 Proteins (Absorbance and Fluorescence)

Key Features: Absorbance

  • Preconfigured and user defined protein types for A280 (quantify purified proteins by absorbance of amino acids at 280nm) and Labeled Proteins (measures abs of protein & labeling dye )
  • Preconfigured Peptide app for easy peptide analysis at A205/215
  • Quick addition of new user defined protein types for improved workflow
  • Ultra short pathlength enables high absorbance capability: up to 1125 mg/ml BSA
  • 11 Preconfigured dyes and fluorophores for Labeled Proteins
  • Option to add an unlimited number of new dyes for quick recall in Labeled Proteins
  • BCA, Bradford, Lowry and Pierce 660 nm colorimetric assays optimized for ease of use
  • Quick save and recall of standard curves available

Key Features: Fluorescence

  • Preconfigured commonly used Qubit® and Quant-it® assays
  • Easy configuration of additional assays
  • Single or multi-replicate standard curve options
  • User control of line fit, trend line analysis, subtraction of blank
  • User control of assay and reported sample concentration units
  • Save and re-use curves and lists of standard concentrations

Advanced App

UV-Vis and Custom user methods

Key Features

Simple but powerful apps enable measurement of more than just nucleic acids and proteins

UV-Vis: Full spectrum analysis:

  • Full spectrum analysis 190 nm – 840nm, reports absorbance up to 6 wavelengths
  • Broad absorbance range for highly concentrated samples (up to 500 A, 1 cm equivalent)

Formula Methods:

  • Add custom formulas to eliminate downstream calculations
  • Apply formulas to absorbance signal at specific wavelengths

Standard Curve Methods:

  • Add new user-defined standard curve methods
  • Quantify any UV-Vis absorbing sample by creating a custom standard curve.

 Basic Fluorometer and Custom Methods – Fluorescence.

Key Features

  • Add new standard curve based fluorescent assays (nucleic assay, protein or other)
  • Basic Fluorometer app returns RFU values for many preconfigured and user-defined dyes/fluorophores

OD 660

  • Bacterial optical density measurements at 600 nm
  • Microvolume shows best performance at high cell densities

Accounts and Data

  • Password protected accounts available
  • All data automatically saved & searchable by account, sample name & type, application and date
  • Configurable reports easily accommodate LIMS and user preferred formats
  • Print microfuge tube labels using a DeNovix supported printer
  • Print reports to a networked printer or DeNovix supported label printer
  • Export full data sets via email; save to a network folder or USB thumb drive.

Software Data Analysis

  •  The DeNovix® DS-11 FX software automatically analyzes and saves all measurement results to the onboard instrument computer.
  • Measurement apps as well as the Data app use a three page structure to maximize the information provided while maintaining an intuitive, uncluttered interface.
  • Absorbance data is analyzed and app specific relevant data such as calculated concentrations and purity ratios are displayed on the Run screen.
  • A running list of sample results are presented on the Results screen.
  • Sample spectra are displayed on the Graph screen. Up to 24 spectra may be overlaid on the graph for quick sample comparisons.
  • Data of interest can be emailed, sent to a network folder or saved to a FAT32 formatted USB thumb drives. The export format is a .csv file that may be opened with common spreadsheet programs such as Excel for user-specific additional analysis. The files include the relevant data presented on the Report screen as well as all absorbance values per wavelength across the measured wavelength range.

DS-11FX Data App

  • The software also includes an intuitive Data app which enables the user to review and export results from multiple measurement sessions at their convenience. This capability makes it easy to compare samples measured on different days.
  • The app includes both Quick and Advanced Search options to make it easy to find your data. Simply filter by account and parameters of interest, tap “Search” and get your results.
Instrument Type Denovix DS 11 FX
Micro Volume Mode
Minimum Sample Size 0.5 µL
Pathlength 0.5 mm (auto ranging to 0.02 mm)
Light Source Pulsed Xenon flash lamp
Detector Type 2048 element CCD
Wavelength Range 190-840 nm
Wavelength Accuracy 0.5 nm
pectral Resolution 1.5 nm (FWHM at Hg 253.7 nm)
Absorbance Precision 0.015 AU (1.0cm), or 1%, whichever is greater
Absorbance Accuracy 1.5% at 0.75 AU at 260 nm
Absorbance Range 0.015 – 750 (1 cm equivalent path length)
Detection limit 0.04mg/ml BSA; 0.75 ng/µl dsDNA
Maximum Concentration 1125 mg/ml BSA; 37,500 ng/µl dsDNA
Lower Sample Surface Material of Construction 303 stainless steel and sapphire window
Upper Sample Surface Material of Construction 303 stainless steel and quartz fiber
Contaminant Detection SmartQC™ Sample Guidance
Fluorometer Mode
Light Sources Blue LED (~470 nm), Green LED (~525 nm),
Red LED (~635 nm), UV LED (~375 nm)
Excitation Filters Blue: 442-497 nm, Green: 490-558 nm,
Red: 613-662 nm, UV: 361-389 nm
Emission Filters Channel 1: 514-567 nm, Channel 2: 565-650 nm,
Channel 3: 664-740 nm, Channel 4: 435-485 nm
Detectors Photodiode, detection range 300-1000 nm
Tube Type 0.5 mL Real Time thin-wall PCR tube (polypropylene)
Detection limit 0.5 pg/uL of dsDNA
Onboard Controller (No PC required)
Operating System Custom Android™ OS
CPU TI OMAP ARM Processor
Display 1280 X 800 high definition color display
User Input Touch screen with swipe and pinch motion; glove compatible
Internal Storage 32GB flash drive
Connectivity 3 USB ports for printer, barcode reader and USB drive
Networking Ethernet and Wi-Fi
General
Measurement Time <4 seconds
Colors Arctic White, Brazilian Blue, Fire Red, Tungsten Silver
Footprint 20 cm X 33 cm
Weight 2 kg
Operating Voltage 12 VDC
Operating Power Consumption 10 W; max 30 W
Approvals CE, UL/CSA, FCC and Japan CAB

denovix-assay-detection

DeNovix dsDNA Assays

  •     Widest Dynamic Range (0.5 pg/µL – 4000 ng/µL)
  •     20X Greater Sensitivity than Qubit®
  •     Integrated into EasyApps® Software
  •     Simple Mix-and-Measure Assay

Assay Choices for Your Application

  • The DeNovix dsDNA Fluorescence Quantification Assays provide the greatest sensitivity and widest dynamic range available today. DeNovix offer three highly specific assay kits for a wide range of sample concentrations. DeNovix kits selectively and accurately measure dsDNA even in the presence of common contaminants such as RNA, ssDNA or protein
  • DeNovix dsDNA assays cover three overlapping ranges of sensitivity. Each kit includes standards for easy set up of a two-point standard curve. DeNovix assays have a wider dynamic range than Qubit®dsDNA assays – and are more affordable. Our breakthrough dsDNA Ultra High Sensitivity assay allows measurement of samples as low as 0.5 pg/µL (original sample concentration), 20X lower than the core range of the Qubit® dsDNA HS assay.

DeNovix dsDNA Broad Range Assay

  • Range: 0.1 ng/µL to 2000 ng/µL (extended to 4000 ng/µL) dsDNA
  • Kit options:  1000 assays, 250 assays or 50 assay evaluation size
  • Excitation:  375 nm      Emission: 435-485 nm

DeNovix dsDNA High Sensitivity Assay

  • Range: 5 pg/µL to 250 ng/µL dsDNA
  • Kit options:  1000 assays, 250 assays or 50 assay evaluation size
  • Excitation:  470 nm      Emission: 514-567 nm

DeNovix dsDNA Ultra High Sensitivity Assay

  • Range: 0.5 pg/µL to 300 pg/µL  dsDNA
  • Kit options:  1000 assays, 250 assays or 50 assay evaluation size
  • Excitation:  470 nm      Emission: 514-567 nm

DeNovix product-family

model-choices  dynamic_range