Magnetic Induction Cycler-MIC-4 qPCR

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The world’s first Magnetic Induction Cycler (4- Channel). The box is small. The ideas are big.

  • Compact, light, fast , precise and wireless.
  • 35 cycles possible in under 25 minutes
  •  Temperature accuracy of ± 0.25ºC
  • Ramp Rates: Heating: 4°C/S, Cooling: 3°C/s
  •  No Calibration required for ever
  •  Available in 2 or 4 channel models (Green / Yellow/Orange/Red)

 

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Speed

  • Mic uses a patented magnetic induction technology to heat your samples and fan forced air for cooling.  This means fast qPCR results through rapid heating and cooling with 35 cycles possible in under 25 minutes*.
  • The Mic real-time cycler is available in 2 or 4 channel models.
  • Each channel uses an independent high intensity LED, photodetector and filter set combining together to give unparalleled detection performance.
  • With a fixed optical path and no moving parts there is never any optical alignment or calibration required.  Even better is that no reference dyes or crosstalk compensation is required.

Accuracy

  • Mic has an aluminium rotor, round and conical, spinning in the induction field.  Mic’s spinning aluminium rotor gives unsurpassed temperature uniformity during dynamic and static operations, there is zero equilibration time. All other block-based qPCR cyclers only promise static uniformity, which could lead to inaccurate data, as samples aren’t heated and cooled at the same rate.
  • Mic’s good to go right out of the box. You don’t need to calibrate – ever.
  • Combine an excellent temperature accuracy of ± 0.25ºC, a first class well-to-well temperature uniformity of ± 0.05ºC, and superior equilibration time uniformity (zero seconds for all wells to reach the same temperature) and you can easily classify difficult class IV SNPs that require melt temperature resolutions of less than 0.1ºC.
  • Excellent reproducibility between samples and repeatability between runs and every Mic qPCR instrument, ensures the highest level of quantification precision; allowing for the detection of two-fold differences in gene expression levels.

Size

  • Mic takes up less space on the bench than your lab book. And weighing in at just 2 kg, this is the most portable compact qPCR cycler on the market. Even four Mic’s stacked together take up less bench space than your current cycler.
  • Only magnetic induction technology by way of its elegant simplicity makes it possible to achieve such a small qPCR instrument footprint.
  • The Mic qPCR tube format uses 0.1 mL strips of four tubes and matching caps, supporting volumes of 10 to 25 µL. A tab on the first tube ensures correct orientation of tubes at all times.
  • High speed centrifugation ensures samples spin down at the start of a run, and the pre-loaded oil overlay inside each tube prevents evaporation and condensation, eliminating the need for bulky heated lids and external centrifuges whilst simultaneously improving the quality of results.
  • These benefits are only possible because of Mic’s unique rotary and magnetic induction cycler technology.

Connectivity

  • Multiple Mics can be operated from one workstation so 48 becomes 96, and 96 becomes 192. Bluetooth technology means fewer cables too.
  • Instruments can communicate via Bluetooth or USB cable and you can connect as many as ten instruments to one PC.  This means up to 480 samples can be analysed simultaneously.
  • Mic’s advanced software has a full suite of qPCR analysis tools to process your sample data.  Individual runs can be collated into a project and analysed together.
  • Naturally you want to be up and running as quickly as possible so installation for the instrument has been made plug-and-play.  It’s as simple as installing the software and starting your first run.

Software

Mic’s software is user-friendly and packed with intelligent features. Designed to meet MIQE specifications,  software offers  the most up-to-date qPCR analysis. With an intuitive interface, setup and analysis is simple, while further control features give you total confidence in your data.

Relative Quantification

  • Mic’s RQ software uses up-to-date mathematical models and well-founded statistical analysis, allowing you to compare gene expression levels for different targets across multiple groups. All the necessary calculations and statistics are carried out within the software. Data is reported both numerically and graphically. With Mic’s superior temperature uniformity detect differences between samples as little as 0.2 fold.

High Resolution Melting

  • Mic’s optional HRM software characterises DNA samples according to their melt behaviour so you can identify mutations, including difficult Class IV SNPs. HRM is the perfect tool for applications including determining allele prevalence, screening for loss of heterozygosity, DNA fingerprinting, DNA methylation, species identification and calculating the ratio of somatic acquired mutations.

Genotyping

  • Use differentiated melt curves from various types of chemistries, including quenched FRET dual hybridization probes, beacon probes or Plexor® to characterize a sample’s genotype. Melt peaks can be inverted to accommodate the different chemistry types.

Absolute Quantification

  • Using a standard curve, AQ allows you to determine the absolute amount of a genetic target. This five point, two fold dilution series produced an efficiency of 98%. The percentage variation between the given and calculated concentration was no greater than 5% allowing for accurate quantification of the unknown sample.
Instrument Type MIC-4
Physical
Dimension Height:130mm (265mm lid open)

Width:150mm

Length:150mm

Weight 2.1kg
Electrical
AC Input: 100-240 VAC, 50/60Hz 4.0 A
Thermal Performance
Temperature Accuracy:

 

± 0.25°C
Temperature Uniformity:

 

± 0.05°C

 

Ramp Rates:

 

Heating: 4°C/s
Cooling: 3°C/s
Temperature Input Range

 

40 – 99°C

 

Optical
Detectors: Photodiode per channel
Excitation Sources: High energy light emitting diode for each channel
Channels:

 

Green: Ex 465nm Em 510nm
Yellow: Ex 540nm Em 570nm
Orange: Ex 585nm Em 618nm
Red: Ex 635nm Em 675nm
Reaction Vessels
Samples per Instrument: 48
Reaction Volume Range: 10 – 25 µL
Operating Environment
Temperature: 18 – 35ºC
Relative Humidity:

 

20 – 80%