G:BOX mini – Multi fluorescence and chemiluminescence

MULTI FLUORESCENCE AND CHEMILUMINESCENCE IMAGING SYSTEM  – DETECTION WITH A DIFFERENCE

  • The G:BOX Mini has 6 or 9 megapixel resolution camera
  • The system as standard comes with a seven position filter wheel and epi white light
  • The G:BOX Mini is capable of many imaging applications including DNA/Protein gels, chemiluminescence, stain free gels and coloured multiplex fluorescence gels.

 

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Overview
  • G:BOX mini is a compact, multi-application imaging system for accurately imaging fluorescence and visible gels, multiplexed fluorescence westerns, stain-free gels and chemiluminescent blots. Fully integrated with single click computer controlled intuitive GeneSys software, you’ll get great results every time.
  • G:BOX mini features the option to use not just white LEDs but multi-colour, blue, green, red and infra-red high intensity LEDs which are up to 200 times brighter than standard LEDs, giving you faster captures and brilliant multiplex fluorescence images.
  • Combining a unique motor driven stage and cooled high resolution 6 or 9 megapixel camera means your G:BOX mini can generate true-to-life, accurate optical images, not just digitally enhanced ones. With a G:BOX mini you’ll see separate close chemi and fluorescent bands or spots even on complex gels and know they’re really part of your data.
  • With our guarantee of free software upgrades not just today but throughout your system’s life, your G:BOX mini will always have the latest imaging capabilities as new techniques come into the working lab.
Features

The G:BOX mini is so versatile that the system can image any of these fluorescence, chemiluminescence and visible applications:

  • Chemiluminescence Western blots
  • X-ray films of chemiluminescent blots
  • DNA or RNA stained with ethidium  bromide, SYPRO or SYBR dyes on  agarose gels
  • Coomassie blue, Texas Red or silver   stained proteins on acrylamide gels
  • Stain-free gels
  • Fluorescent blots stained with Qdots,  DyLight, Alexa Fluor, Cy Dyes, and IR dyes
  • GFPs
  • Colonies or plaques on agar plates

POWERFUL

  • Built on the successful G:BOX range, the G:BOX mini is a compact, multi-application powerhouse for accurately imaging fluorescence and visible gels, multiplexed fluorescence westerns, stain-free gels and chemiluminescent blots.  Fully integrated with single click computer controlled intuitive GeneSys software, you’ll get great results every time.

FAST

  • G:BOX mini features the option to use not just white LEDs but multi-color, blue, green, red and infra-red high intensity LEDs which are up to 200 times brighter than standard LEDs, giving you faster captures and brilliant multiplexed fluorescence images.

ACCURATE

  • Combining a unique motorized stage and cooled, high resolution 6 or 9-megapixel camera means your G:BOX mini can generate true-to-life, accurate optical images, not just digitally enhanced ones.  With a G:BOX mini you’ll be able to distinguish between close chemi and fluorescent bands or spots even on complex gels and know they’re really part of your data.

FUTURE-PROOF

  • With our guarantee of free software upgrades not just today but throughout your system’s life, your G:BOX mini will always have the latest imaging capabilities as new techniques come into the working lab.

Right lighting, right application

  • White light
    To position your samples, see visibly stained blots and colored markers on Westerns, the G:BOX mini comes with overhead environmentally friendly, long-life white LED EPI lighting.
  • HI-LED EPI lighting options
    When you want to image multiplex fluorescent gels and blots, the G:BOX mini can be configured with the option of HI-LED RGB, RIR or RGBRIR. HI-LEDs are up to 200 times brighter than standard LEDs, giving you faster exposure times and great images in just one click, making the G:BOX mini an unrivalled, cost-effective alternative to laser-based technology. Using these high powered HI-LEDs fitted with special narrow band pass filters allows you to accurately image multiplexed blots/gels with minimal ‘cross-talk’ between dyes.
  • UV transilluminator option
    If you simply need to see ethidium bromide stained DNA gels and stain-free protein gels then opt for the slide in and out, easy access 302nm UV transilluminator. 254nm and 365nm wavelengths are also available.
  • Visible transmitted light options
    For viewing Coomassie Blue, silver stain and other visible stained gels, a conversion screen which you can place over the UV transilluminator to produce a large, evenly illuminated white light is available.
  • Blue light conversion screen
    For viewing ‘safer’ fluorescent dyes such as SYBR Safe, you can choose to illuminate with the optional blue conversion screen which sits over the UV transilluminator producing trans blue illumination at 465nm.
  • Blue light transilluminator option
    For visualising many fluorescent dyes including ethidium bromide and the safe dyes without using UV, you can choose the 470nm UltraSlim Blue LED transilluminator.
Why Buy

Why buy this product

  • Luxury lens
    Great images start with a great lens and this F/0.95 motor driven lens with data feedback and automated focus is the best. Using GeneSys software, the G:BOX mini easily controls lens aperture size, focus and zoom to get the results you want to see.
  • High resolution cameras
    Choose between a 6 or 9 megapixel resolution camera. These work hard over a range of wavelengths to ensure you’ll separate those close fluorescent bands and spots even on 2D gels.
  • Super low cooling
    Superior Peltier cooling of the camera lets you increase exposure times to detect your faint chemiluminescence without adding annoying background noise.
  • Filter choice
    A 7 position motor driven filter wheel controlled by GeneSys software allows you to add the filter for the fluorescent stain you like to work with. Since imaging ethidium bromide and SYBR(R) stained DNA gels are common, we’ve even included a UV filter to get you started.
  • Real imaging stage
    When you’re working with smaller and low light emitting gels and blots, an automatic motor driven stage with automated focus is brilliant because it lets you get your samples closer to the camera, generating true to life optical images and not just digitally enhanced copies.
  • Total control
    Easily integrating a G:BOX mini to your choice of PC and printer gives you greater flexibility than using an integrated tablet, allowing you to run the GeneSys touch screen controls on a large screen, store a huge number of images and rapidly print low resolution all the way up to publication
    quality pictures.
  • G:BOX mini mount option
    The G:BOX mini systems are now available with a cleverly-designed screen mount option. This sits neatly on top and lets you safely secure the screen to control the system or view gel and blot images. The mount does not come with a screen/monitor.
Software

CAPTURE, STORE & MANIPULATE IMAGES WITH GENESYS IMAGE ACQUISITION SOFTWARE

GENESYS SOFTWARE LOAD AND GO IMAGING

  • The G:BOX mini is powered by GeneSys ‘application driven’ image capture software containing an extensive database of dyes, stain-free options and imaging protocols.  For quick and easy imaging with a G:BOX mini, all you need to know is the size and type of gel or blot you’re using and GeneSys automatically selects the right lighting, filters and focus for you to get the perfect image.
  • It has a simple icon based user interface usable on Win10, 8, 7 and XP platforms.
  • VERSATILE MULTIPLEXING: Using GeneSys in the G:Box mini you can image up to four different fluorophores per experiment and you can choose to see them as a multi-color image, as a color overlay or as single images making it easier for you to find the information you want from your gel or blot.
  • CUSTOMIZABLE SETTINGS: If you prefer to choose your own settings, you can even use GeneSys in manual mode. Alternatively, if you’re running several repeat applications and want to automate the image capture, you can save a protocol of sample type, dyes, lighting, filters, focus and sample size to set up one button quick image capture.
  • SENSITIVE WESTERNS: When you’re imaging low light chemiluminescence Western blots, you can use the GeneSys binning feature to reduce exposure times or increase sensitivity. Binning combines pixels into 2×2, 3×3, 4×4, 5×5 or 6×6 formats to produce a “super pixel” which collects more light, increasing sensitivity or speeding up image capture time. GeneSys also lets you generate one image or a series of timed images of your Westerns. You can even image visible/rainbow molecular weight markers and automatically overlay them on your chemiluminescent image making sure that you have perfect Western blot images every time.
  • PICTURE PERFECT: The G:BOX mini comes with a calibrated camera which automatically eliminates hot pixels or imperfections, generating a clear background free from ‘speckles’ or ‘spots’. The GeneSys software includes Dynamic Fielding to automatically correct white light shadows, producing a perfect ‘flat’ background and auto gamma control to automatically set the black and white levels, improving definition between bands or spots and image background. The high resolution 6 or 9 MP cameras will produce superb, publication ready pictures of your results and can save as proprietary SGD or TIFF, JPEG or BMP formats.
  • TIME-SAVING MULTIPLEXING: Using a G:BOX mini you can capture a broad dynamic range of fluorescence, giving you exceptional linearity and accurate quantification. The GeneSys software helps you easily detect up to four different fluorophores on the same gel or blot and automatically overlays data from each channel, while letting you view individual channels to see where bands overlap. You can normalize band intensity values to another protein or loading control so you can save time by using the same blot without having to strip and re-probe.
  • ENHANCING YOUR MULTIPLEXING IMAGING CAPABILITIES: Using a combination of different fluorescent dyes in conjunction with our excitation light sources and filter combination will allow you to multiplex up to four colors, permitting you to study several proteins on the same blot
  • SMART CHEMILUMINESCENCE: When you’re imaging chemiluminescence blots it’s often difficult to get the right exposure time. Using GeneSys, you can set the G: BOX mini to give you the optimum exposure depending on whether you want a quick or a high-quality image. Since the dynamic range of the G:BOX mini is better than X-ray film you’ll get more accurate quantifiable data too. You can even capture images of visible protein markers and using GeneSys you can overlay them automatically on your chemiluminescent image to make your molecular weight calculations easier.
  • All G:BOX chemi  systems are stain-free ready (Stain-free imaging Stain-free technology) and with GeneSys software it has never been easier to capture perfect images of your stain-free gels.
  • GeneSys has a number of automatic functions which help the image capture process to produce perfect, high quality images. (Effective resolution, Flatfix technology, Neutral fielding, Auto gamma control)
  • GeneSys allows the user to take the guess work out of imaging, and produce quick and simple publication quality images.
  • All files produced are a unique SGD format, where only raw data is analysed for quantification of samples.
  • Files can also be saved as TIFF, JPEG, BMP formats.
  • User protocols can be saved to review previous images captured, and easily replicate future imaged gels.
  • Neutral fielding for correction of uneven background illumination
  • All captured images can be simply edited with annotations, edit tools, image sharpen and exported to GeneTools

ANALYSE, DOCUMENT & QUANTIFY GELS WITH GENETOOLS  ANALYSIS SOFTWARE.

  • 1D gel analysis •MW/BP calculation •Quantification •E-gels •Band matching with dendrograms •Spot blots •Colony counting.
  • Band analysis/densitometry
    ID analysis at the ‘click’ of a button. Ideal for 1D analysis, the software has automatic lane and peak detection. GeneTools software automatically compensates for smiling or distorted bands and tracks. Molecular weight or base pair values can be calculated using standards from one or more tracks. Quantification can also be automatically calculated from standards in a number of ways. Detailed results can be instantly printed or transferred to Word or Excel.
  • Band matching
    Band matching can be performed by position, defined Rf or calibrated molecular weight/base pair value.
  • Automatic molecular weight/base pair calculations for sample bands based on standards from one or more lanes
    GeneTools has a library of markers for automatic or manual assignment which are user configurable.
  • Multi-tier analysis for high throughput screening
    Especially useful for analysing high throughput gels such as E-Gel (Invitrogen, UK). GeneTools software provides multi-well line functions with automatic track alignment.
  • Manual band quantification
    The user can manually quantify bands, spots or other objects on a sample. All data is instantly exportable to Microsoft Excel.
  • Spot blot analysis
    Automatic detection of spots using a spot grid, quantitative and incidence analysis. The software can also perform multiple background subtraction methods.
  • Colony counting
    Automatic counting of two colour colonies with the ability to count light and dark colonies on the same plate.
  • Export results to Microsoft Excel and Word
    Customised results tables can be exported to either Microsoft Word or Excel.
  • Report generation
    Saves reports with customised protocols  designed by the user. All reports are compliant with CRF 21 Part 11.
Specifications

Detection with a difference

SYSTEM G:BOX mini 6 G:BOX mini 9
Image resolution (megapixels) 6 9
Effective resolution (megapixels) 18 27
A/D 16 bit 16 bit
Greyscale 65,536 65,536
Quantum efficiency (@425nm) 73% 73%
Lens (motor driven) F0.95 F0.95
Motor driven stage True optical imaging True optical imaging
Filter wheel (7 position motor driven) Yes Yes
UV filter Yes Yes
Use with external PC and printer (not included) Yes Yes
LIGHTING
Epi LED white lights Yes Yes
HI-LED (red, blue, green) Optional Optional
HI-LED (red, infrared) Optional Optional
HI-LED (red, blue, green, infrared) Optional Optional
Visible light converter Optional Optional
Blue converter screen Optional Optional
Slim slide-out UV transilluminator (20cm x 20cm) Optional – 254, 302, 365nm wavelengths Optional – 254, 302, 365nm wavelengths
UltraSlim Blue LED transilluminator
Gel size (cm)
Optional

10 x 12

Optional
10 x 12
DIMENSIONS
Max image area (cm) 15 x 12 15 x 12
Min image area (cm) 10 x 8 10 x 8
W x H x D (cm) 40 x 64 x 52 40 x 64 x 52
Weight (kg) 40 40
Power input (V) 100-240 100-240
Models

Products

Advanced gel doc systems

Applications GeneGnome XRQ G:BOX Chemi XRQ G:BOX Chemi XX6 G:BOX Chemi XX9 G:BOX mini
Fluorescence UV transilluminator Option Option Option Option
Fluorescence UV Epi Option Option Option
Blue light Option Option Option Option
Visible stain converter Option Option Option Option
Colony plate Option Option Option Option
Spot blot Option Option Option Option
Fluorescence (colour) Option Option Option Option
Chemiluminescence
Fluorescence IR Option Option Option
Proteomics (2D) Option Option Option